Journal: Scientific reports

Article Title: Developmental exposure to perfluorooctane sulfonate(PFOS) impairs the endometrial receptivity.

doi: 10.1038/s41598-024-84732-2

Figure Lengend Snippet: Fig. 5. Effect of PFOS on endometrial receptivity. (A) Molecular docking of PFOS with HOXA10 and ITGB3. (B) The mRNA expression levels of HOXA10, ITGB3 were detected by using RT-qPCR. (C-D) Expression and quantification of HOXA10, ITGB3 and FOXO1 proteins in cells. Mitochondrial damage (red arrow) in endometrial stromal cells. Immunofluorescence staining reveals expression of HOXA10 (E), ITGB3 (F) and FOXO1(G) in endometrial stromal cells. (H) Immunofluorescence statistical analysis. (I) Transmission electron microscopy showed the normal mitochondrial morphology (green arrow), Scale bar = 200 μm. Results are presented as the means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: The membrane was incubated with primary antibodies including GAPDH, Bax, Bcl2, HOXA10, ITGB3, FOXO1, cytokeratin 18, and vimentin from Proteintech (China).

Techniques: Expressing, Quantitative RT-PCR, Immunofluorescence, Staining, Transmission Assay, Electron Microscopy

Journal: Chemistry of Materials

Article Title: Importance of Molecular and Bulk Dynamics in Supramolecular Hydrogels in Dictating Cellular Spreading

doi: 10.1021/acs.chemmater.3c01676

Figure Lengend Snippet: Figure 3. Mechanical and dynamic characterization of soft [G′ ∼100−200 Pa, (A−F)] and stiff [G′ ∼1 kPa, (G−L)] supramolecular UPy and BTA hydrogels. (A,G) Representative angular frequency sweep plot at 1% strain, (B,H) representative strain sweep plot measured at 1 rad/s both showing the storage (G′) and loss modulus (G″), in (C,I) G′ quantified, in (D,J) the loss factor (tan(δ)) showing the ratio of viscous to elastic response, in (E,K) a representative stress relaxation plot measured at 7.5% strain and a strain rise time of 0.01 s, and in (F,L) the released stress quantified after 1000 s.

Article Snippet: The samples of interest were incubated with antihuman, mouse primary antiintegrin β1 antibodies (from Santa Cruz; sc-53711, 1:100 dilution with a final concentration of 2 μg/mL) or with antihuman, mouse primary β3 antibodies (from Biorbyt; orb248939, 1:100 dilution with a final concentration of 2 μg/mL) in PBS containing 3% BSA at 4 °C overnight.

Techniques: